Gel diffusion method for determining the titer of duck hepatititis virus.

Application of polymerase chain reaction in detection of egg drop syndrome (EDS) virus

  Egg drop syndrome (EDS) is caused by a hemagglutinating adenovirus which has become a major cause of lost egg production and sever economic losses in breeder and layer chicken flocks throughout the world. A PCR assay was optimized for detection of EDS virus in inoculated allantoic fluids (AFs) of duck and chicken emberyonated eggs. Two strains of EDS viruses were propagated in allantoic cavit...

Study on hepatitis B virus infection using electron microscopy and gel diffusion techniques

A Novel Spot-Enhancement Anisotropic Diffusion Method for the Improvement of Segmentation in Two-dimensional Gel Electrophoresis Images, Based on the Watershed Transform Algorithm

Introduction Two-dimensional gel electrophoresis (2DGE) is a powerful technique in proteomics for protein separation. In this technique, spot segmentation is an essential stage, which can be challenging due to problems such as overlapping spots, streaks, artifacts and noise. Watershed transform is one of the common methods for image segmentation. Nevertheless, in 2DGE image segmentation, the no...

Effect of in vitro adaptation of Marek's disease virus on pock induction on the chorioallantoic membrane of embryonated chicken eggs.

Cell-associated preparations of several isolates of Marek's disease virus produced more pocks on the chorioallantoic membrane of embryonated chicken eggs than plaques in duck embryo fibroblasts, thus indicating that lesion response in eggs was more sensitive than cytopathic response in duck embryo fibroblasts for assaying low-passage Marek's disease virus. Adaptation of the virus to cell cultur...

Diagnosis and characterization of duck beak atrophy and dwarfism syndrome in Chongqing of China

Duck beak atrophy and dwarfism syndrome (BADS) is a newly emerged disease in China since 2015. In October 2017, an unidentified disease occurred in Cherry Valley ducks, Chongqing municipality, the southwest of China. The affected birds showed short beak and growth retardation clinical signs. The disease caused approximately 20.00% morbidity and serious weight loss due to retarded growth. In ord...